Understanding How To Use The C-Kit Antibody And Versions Available

The C-kit Antibody comes in a monoclonal and polyclonal variety. Both versions come in sizes of 0.1 ml, 0.5 ml, 7.0 ml, and 1.0 ml and are designed for research only use. The Monoclonal version has a clone of SP26 while the polyclonal version has none. Both are human tested and recognizes the 145kDa protein, which is also identified as CD117/p145kit.

It will not interfere with the bonding of the SCF to the C-Kit antibody and precipitates the unoccupied and occupied form of the C-Kit. It can stimulate multiple biochemical responses in migration, survival, and proliferation at the cellular level by binding the stem cell factor with tyrosine kinase (Type III).

It also plays a necessary role in melanogenesis, hematopoiesis, and gametogenesis. Likewise, it is a cytokine receptor for hematopoietic stem cells and others. Mutations of the gene can be associated with mast cell disease, chronic myelogenous leukemia, and gastrointestinal stromal tumors.

Applications And Procedures

It is designed to be used for IHC (Immunohistochemistry), and both versions can be used with paraffin-embedded and formalin-fixed tissues. Dilution options are different for both versions of the antibody. For example, the monoclonal version must be diluted at a 1:50 ratio while the polyclonal version is diluted at a ratio of 1:100.

Incubation periods are also different, as the monoclonal version must be incubated for 30 minutes and the polyclonal for 10 minutes.

The positive control for both versions is the human gastrointestinal stromal tumor, though the polyclonal version can also have the tonsil and skin as a positive control. Likewise, either version should be stored at two to eight degrees Celsius and shouldn’t be frozen.

The C-Kit antibody is an excellent product that can help determine gene mutations and more. Visit Spring Bioscience at www.springbio.com to learn more or to purchase this product.

1 person likes this post.

Leave a Reply

Your email address will not be published. Required fields are marked *

12 − eleven =